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KMID : 0545119970070060417
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Journal of Microbiology and Biotechnology
1997 Volume.7 No. 6 p.417 ~ p.422
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Enhanced Production of Maltotetraose-producing Amylase by Recombinant Bacillus subtilis LKS88 in Fed-batch Cultivation
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Kim, Dae Ok
Park, Kyung Moon/Song, Jae Wook/Seo, Jin Ho
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Abstract
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Recombinant Bacillus subtilis LKS88[pASA240] containing the amylase gene from Streptomyces albus KSM-35 was exploited in fed-batch cultivation for mass production of maltotetraose-producing amylase. The effects of dissolved oxygen, additional organic nutrients (peptone and yeast extract) and mixed carbon sources (glucose plus soluble starch) on amylase production were examined in fed-batch operations in an effort to determine the optimum condition for a maximum amylase productivity. Under the optimum conditions, maximum amylase activity was about 42 times higher than that obtained in batch cultivations, indicating that mass production of maltotetraose-producing amylase could be accomplished in fed-batch cultivation of the recombinant B. subtilis strain.
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